Reporting NADPH fluxes

Reporting NADPH fluxes

The NADP+/NADPH coenzyme couple powers cellular biosynthesis and oxidative defense. A new study tracing glucose-derived deuterium during proline biosynthesis analyzes subcellular perturbations in NADPH utilization, revealing that NADP+/NADPH coenzyme pools in the cytosol and mitochondria are regulated independently.