We interrogate the metabolome with unprecedented depth,
confidence, and quantitative accuracy by exploiting an
armamentarium of advanced analytical approaches.
Technological Innovation
Our workflows use in-house informatics solutions, home-built equipment, and instrumentation from vendors including:
QTOF and Orbitrap Mass Spectrometers
Quadrupole-time-of-flight and Orbitrap instruments provide high-resolution data that can be used to profile water-soluble metabolites and lipids at the comprehensive scale.
Ion Mobility Spectrometry
Compound identification is enhanced by matching collisional cross sections to authentic standards.
Mass Spectrometry Imaging
In situ metabolomics takes advantage of technologies such as MALDI, DESI, and NIMS to localize compounds to specific anatomical regions or cells within a tissue.
High-Resolution Fluorescence Microscopy
The Leica Thunder imager uses a new Instant Computational Clearing method to generate high-resolution and high-contrast images for 3D visualization of biological processes in cells and tissues.
In-House Zebrafish Facility
A zebrafish Tecniplast system is used to house genetic models of cancer and enable in vivo isotope tracing
Respirometry
Seahorse extracellular flux analyzers provide insight into oxygen consumption and other discrete changes in bioenergetics in real time.
In vivo imaging system
The Pearl Triology imaging system enables bioluminescence detection and dual-channel near-infrared fluorescent detection, which we use for monitoring the location, progression, and therapeutic response of tumors in mice.
